全文获取类型
收费全文 | 48194篇 |
免费 | 6788篇 |
国内免费 | 19176篇 |
出版年
2024年 | 122篇 |
2023年 | 1264篇 |
2022年 | 2064篇 |
2021年 | 2707篇 |
2020年 | 2399篇 |
2019年 | 2818篇 |
2018年 | 2209篇 |
2017年 | 1896篇 |
2016年 | 2217篇 |
2015年 | 2989篇 |
2014年 | 4055篇 |
2013年 | 3709篇 |
2012年 | 5046篇 |
2011年 | 4857篇 |
2010年 | 3683篇 |
2009年 | 3736篇 |
2008年 | 4119篇 |
2007年 | 3848篇 |
2006年 | 3548篇 |
2005年 | 3059篇 |
2004年 | 2359篇 |
2003年 | 2081篇 |
2002年 | 1833篇 |
2001年 | 1670篇 |
2000年 | 1505篇 |
1999年 | 1034篇 |
1998年 | 507篇 |
1997年 | 370篇 |
1996年 | 254篇 |
1995年 | 284篇 |
1994年 | 199篇 |
1993年 | 174篇 |
1992年 | 201篇 |
1991年 | 184篇 |
1990年 | 153篇 |
1989年 | 125篇 |
1988年 | 113篇 |
1987年 | 105篇 |
1986年 | 93篇 |
1985年 | 118篇 |
1984年 | 78篇 |
1983年 | 55篇 |
1982年 | 92篇 |
1981年 | 34篇 |
1979年 | 20篇 |
1978年 | 16篇 |
1977年 | 19篇 |
1976年 | 15篇 |
1975年 | 14篇 |
1972年 | 15篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
1.
2.
Ji Guo Su Xiao Ming Han Xiao Zhang Yan Xue Hou Jian Zhuo Zhu Yi Dong Wu 《Journal of biomolecular structure & dynamics》2016,34(3):560-571
Protein collective motions play a critical role in many biochemical processes. How to predict the functional motions and the related key residue interactions in proteins is important for our understanding in the mechanism of the biochemical processes. Normal mode analysis (NMA) of the elastic network model (ENM) is one of the effective approaches to investigate the structure-encoded motions in proteins. However, the motion modes revealed by the conventional NMA approach do not necessarily correspond to a specific function of protein. In the present work, a new analysis method was proposed to identify the motion modes responsible for a specific function of proteins and then predict the key residue interactions involved in the functional motions by using a perturbation approach. In our method, an internal coordinate that accounts for the specific function was introduced, and the Cartesian coordinate space was transformed into the internal/Cartesian space by using linear approximation, where the introduced internal coordinate serves as one of the axes of the coordinate space. NMA of ENM in this internal/Cartesian space was performed and the function-relevant motion modes were identified according to their contributions to the specific function of proteins. Then the key residue interactions important for the functional motions of the protein were predicted as the interactions whose perturbation largely influences the fluctuation along the internal coordinate. Using our proposed methods, the maltose transporter (MalFGK2) from E. Coli was studied. The functional motions and the key residue interactions that are related to the channel-gating function of this protein were successfully identified. 相似文献
3.
4.
5.
建立一种靶点蛋白质快速定量检测方法。在原有侧向流动免疫层析技术的基础上,通过优化层析材料和纳米微球的均一性、改进检测区的检测方法,经逐点扫描技术,建立标准浓度曲线,以达到对临床靶点蛋白质的定量检测。以乳腺癌组织中的Her2表达为例,通过对已知浓度样品的检测,验证本技术方法的准确度大于96%。另外,以蛋白质免疫印迹作为组织中特定蛋白质检测金标准,分析临床肿瘤组织中Her2蛋白的含量,其准确率也达到95.5%,而免疫组织化学方法检测准确率仅为69.58%。新型免疫层析法检测结果与靶向治疗患者的愈后密切相关(P<0.01)。改进后的新型免疫层析方法能够准确地对临床靶点蛋白质进行定量检测,而且结合侧向流动技术的简单、快速和易用性,这种新型检测方法可以广泛应用于临床组织标本、血液标本和体液标本中靶点蛋白质的临场定量检测,在一定程度上可以替代免疫组化技术。 相似文献
6.
Robert M Bennett Ronald Friend Kim D Jones Rachel Ward Bobby K Han Rebecca L Ross 《Arthritis research & therapy》2009,11(4):R120-14
Introduction
The Fibromyalgia Impact Questionnaire (FIQ) is a commonly used instrument in the evaluation of fibromyalgia (FM) patients. Over the last 18 years, since the publication of the original FIQ, several deficiencies have become apparent and the cumbersome scoring algorithm has been a barrier to widespread clinical use. The aim of this paper is to describe and validate a revised version of the FIQ: the FIQR. 相似文献7.
8.
9.
10.
A haploid callus line from anther cultures of the Asiatic hybrid lily ‘Connecticut King’ was maintained for a long term. The
survival and growth of the haploid calluses were affected by auxins of picloram, α-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic
acid (2,4-D) and temperatures of 25, 15 and 7 °C during culture. Picloram was more suitable for maintenance of the haploid
calluses, whereas NAA and 2,4-D led to root and shoot formation from the haploid calluses. The best temperature for maintenance
was 25 °C. About 90% of cells in calluses were maintained in haploid level during 60 weeks of subculture, and about 80% of
cells were haploid in the calluses maintained over 2 years with the MS medium containing 4 μM picloram in the dark at 25 °C.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献